Isle of May 2017 – The last PHATS field season begins

The PHATS team 2017 geared up to head out to the island

The PHATS team is back out on the Isle of May! For the rest of the year, we’ll be out here studying the breeding grey seals and how the physiology of fat tissue in wild animals is affected by persistent organic pollutants (POPs). This year we have 4 team members; Dr Kimberley Bennett and her new PhD student Laura Oller have come from Abertay University, Holly Armstrong has come up from Plymouth University and I’ve come from the Sea Mammal Research Unit at the University of St Andrews.

Driving the lab gear out to the Isle of May

This year the field season has had a rather unconventional start, as for the first week of the breeding season Dr Bennett and I were away in Canada for the 22nd Biennial Marine Mammal Conference (more on this later). So back in mid October we had to do an early provision run to get most of the laboratory gear we need for the 2 month long field season out onto the island. The other field teams working on the seals then arrived while we were still in Canada, and when we finally arrived last week we had to hit the ground running as there were already lots of potential study animals for the PHATS project. Even though we’ve only been on the island for three days, we’ve already found the first five seals for this year’s study cohort, who have duely been named Alpha through to Echo from the phonetic alphabet. We’ll be here until mid december to try and collect all the data we need to finish the PHATS study, as this is the last field season that is planned for the project.

A 1-2 day old grey seal pup on the Isle of May, with a still healing umbilical cord

Outside of the lab we’ve set up on the island, the breeding season is in full swing for the grey seals that have come here to give birth and mate. The number of mother-pup pairs is steadily rising and the large males are already starting to battle for position among the females. There aren’t too many weaned pups around yet, but within a few weeks there will be loads all around the edges of the colony as their 18 days with their mothers comes to an end and the females return to sea, leaving their pups to fend for themselves. Every week I’ll write about a different aspect of the breeding colony and the PHATS project for this blog, with updates on how our study seals are doing and what the field team are getting up to. You can check back here or find me on twitter for updates.

Male grey seals fighting on the Isle of May colony, biting another seal’s hind flippers while they run away appears to be the ultimate insult!
Giving my talk at the marine mammal biennial in Halifax, Canada

MEANWHILE as mentioned above, team leader Dr Bennett and I have been travelling, heading to Halifax, Canada for the 22nd Biennial Marine Mammal Conference to present the findings of the PHATS project so far. It’s always fantastic to get to meet up with fellow marine mammal scientists, hear what discoveries have been made in the last 2 years and show people what you’ve been working on. I also go to take part in a workshop dedicated to a subject that’s become especially important to me, marine mammal endocrinology. It was great to meet all the other people working on the challenging topic of marine mammal hormones, and to hear about the inventive ways people get around working with tricky species like whales out at sea. I’ve had a pretty busy year for conferences in 2017, hopefully we’ll keep finding out new, interesting things from both my PhD on oxytocin and from the PHATS project so we can go to some more next year!

Dr Bennett and I exploring the coast of Canada near Halifax

 

 

The SOI Early Career Network, October talks and upcoming Isle of May field season

Pregnant female grey seals and yearlings hauled out on the rocks around the Isle of May, Scotland

It’s that time of year once again, autumn is here and that means I’m making inventories and packing equipment for the PHATS team’s field season on the Isle of May. We’ll be heading out to the island at the end of October to begin our last data collection season for the project, and we’ll be living on the island and studying the grey seals until mid December. Before we head out though I’ve got a busy month ahead of me, as I’ll be presenting PHATS work, my PhD work on oxytocin and talking to the public about grey seals. But before we get onto where and when I’ll be presenting, I’m quickly going to give a shout out to a new group I’ve been involved with setting up over the last few months, the SOI Early Careers Network.

This grew out of a group of friends from the Scottish Ocean’s Institute (SOI) meeting to help each other practise for presentations, to give feedback on each other’s ideas and to chat and share resources about the various issues early career scientists face. We then decided to open the gatherings up to any early career researcher at the SOI, and the group has grown ever since. We meet at least every week, sometimes more, to discuss anything our members currently need help or advice with. Right now we are having lots of conference poster and talk preparation sessions with the biologging meeting and the marine mammal biennial happening in September and October. We’ve also discussed loads of topics including statistical methods, funding awards and public outreach.

If you are an early careers researcher at the SOI you are very welcome to join us, our meetings aim to address whatever our members feel they currently need, providing a responsive support system with a relaxed, friendly environment. Please visit our new website here to find out more, see when our next meetings are and sign up to the mailing list, or you could come along to our welcome day event on Tuesday 3rd October (next week) to meet some of us and chat about the group and early career life.

A SOI early career network meeting for practising talks and sharing presentation ideas

I’ll certainly be practising the various presentations I need to give in the coming month at the ECN! I’ve already been to one conference this month, the wonderful meeting of the British Neuroendocrinology Society in Nottingham where I got to present my work on oxytocin and behaviour in seals. Next I’ll be talking to the public about any and all aspects of grey seal life on the Isle of May, during their annual seal weekend. This happens to celebrate the start of the grey seal breeding season, and afterwards the island is then closed to the public for the rest of the year to protect the breeding seals from disturbance.

Presenting my oxytocin work at the BNS 2017 conference in Nottingham

I’ll then be travelling to the University of Edinburgh to talk about my work on oxytocin and behaviour on the 10th October. I can’t wait to meet everyone at the Centre for Integrative Physiology and hear all about their amazing research on neuroendocrinology, I got to meet a few lab members at the BNS 2017 conference and their studies on modelling oxytocin dynamics are fascinating. Finally I’ll be heading out to Canada towards the end of October to present our PHATS work at the 22nd Biennial Conference on Marine Mammals. Phew, it’s going to be a crazy month! If you’d like to know more about any of my work, feel free to say hi at any of these events, or you can keep up with me on Twitter (@KJRscience).

EVENTS:

1st October – Isle of May seal weekend

10th October – 3pm talk at the Centre for Integrative Physiology, University of Edinburgh

22nd-27th October – 22nd Biennial Conference on Marine Mammals, Halifax, Canada

Hauled out grey seals in East Tarbet in the north part of the Isle of May, Scotland

 

New Publication – An explant approach to studying fat tissue function in wild animals

Adult male grey seal hauled out on a rocky seashore. Even in wild conditions, the PHATS team is bringing cell culture into the field!

Link to article: https://www.nature.com/articles/s41598-017-06037-x

Or read the summary here on this site.

Behaviour 2017’s fantastic closing dinner party, complete with a live band featuring 6 ukuleles!

I have now returned from an incredibly successful week at Behaviour 2017 (link), and what a spectacular conference it was! The sheer variety of science that people were talking about was incredible and inspiring, plus I got a great response to both my symposium talk on seal oxytocin and the poster I presented on aggression. I meet so many wonderful people, heard lots of interesting talks and I even managed to avoid getting roasted in the blazing Portuguese sun! I had never previously been to a behaviour conference of any kind, but this one has really encouraged me to keep an eye out for future ASAB meetings to present at. Huge thanks to the lovely people working as part of the SoHaPi research group for inviting me to speak at your symposium, I look forward to meeting up with you all in the future!

Taking the stage at Behaviour 2017 to talk about my work on oxytocin in wild seals

More good news was waiting for me when I arrived home from Portugal; our PHATS team leader, Dr Kimberley Bennett, let us know that the first paper the PHATS team have worked on was coming out at last! This paper details our work investigating whether an explant approach (basically blobs of many living cells) would work for culturing fat (or adipose) cells collected from wild animal species in field conditions. Additionally, we wanted to know whether we could manipulate the explants during culture to

100mg explants of adipose tissue weighed out and ready for transfer to culture plates for their 24 hour exposures to different treatments on the Isle of May, Scotland.

uncover the physiological consequences of changes in the nutrients or hormones the cells have access to. We found we could not only keep our cells alive once collected from wild seals on the coast of Scotland, but once transported back to the lab we could culture the cell explants for at least 24 hours. During this time we could expose the adipose cells to different treatments, such as high glucose concentrations in the cell culture media (the sugary, salty goo that cells are suspended in during culture to keep them alive) or difference hormone additions, such as hydrocortisone. We found significant differences in the metabolic profiles of adipose cells given different treatments, demonstrating this technique could be used to test the responses of wild animal tissue to a variety of substrates an individual may physiologically generate, or be exposed to.

Studying wildlife physiology is always challenging because collecting samples is tricky, typically giving small samples sizes in less than ideal conditions for complex labwork. However our work to bring cell culture techniques to the wild regions of Scotland shows that even difficult processes like cell culture, which require sterile conditions, aseptic technique and specialised equipment, are possible with thought and preparation beforehand. Studying cell function in wild animals is important as how different tissues function in response to different environmental challenges will impact on how individuals survive. Fat tissue is especially crucial for survival as it represents the energy stores animals have to rely on when conditions are tough, and also helps keep individuals warm in cold environments. By understanding how fat tissue functions, we can better understand why different species in changing environments can either adapt to meet new energetic challenges or be overwhelmed by them.

Even in muddy, windy or wet conditions, cell culture experiments can be possible if you are careful! (grey seal mothers and pups on the Isle of May, Scotland)

Speaking of ‘the wild regions of Scotland’, it’s that time of year when I start prepping all the field equipment for the PHATS team’s annual research trip to the Isle of May grey seal breeding colony, off the east coast of Scotland. Join us here for our fieldwork blog, bringing you all the adventures we have running a tissue culture lab on an island full of seals. We are scheduled to leave in late October, and will stay on the island studying the seals for about 2 months, heading home just in time for Christmas (hopefully!). I’ve also got two more conferences to attend before I go off into the field, one in September in Nottingham, UK with the British Society for Neuroendocrinology and one in October in Halifax, Canada with the Society for Marine Mammalogy. If you are going to either and want to say hello I look forward to meeting you there!

Weaned grey seal pups occupying the path down to Kirkhaven harbour on the Isle of May, Scotland

Attending Behaviour 2017 and other upcoming conferences

Hear about all the hormone, behaviour and adipose tissue function work I’ve done with seals at any of the three conferences I’m attending this year!

I’m going to Behaviour 2017 in Portugal next week!

Conference information: link

I’m going to be talking about my work on oxytocin and maternal and social behaviour in grey seals on monday afternoon, plus I’m presenting a poster on the development of aggressivness in seal pups on wednesday and thursday.

Symposium talk: Syposium 1 on Monday 31st July at 17:35 – 18:05

The symposium is titled ‘How Social Behaviour can impact individual health and fitness’. It will feature talks looking at how social living can impact on a range of aspects of an individual’s physiology, and the potential fitness costs and benefits associated with them. The talks cover primate species, fish and of course seals in my case!

Poster: Poster 278, Wed + Thurs, 2nd-3rd August 14:00 – 16:00

If you’re going to the conference and would like to find out more about my work it would be great to meet you there!

Please do say hello if you would like to talk to me about my research, my crazy ginger hair usually make me easy to find!

I’m also attending two other conferences this year, one to (hopefully) talk about my oxytocin work and the other to talk about the tissue culture work I’ve done

(TBC) Oxytocin work – 10th – 12th September
British Society for Neuroendocrinology, Nottingham (conference site: link)

Tissue Culture work – 22nd – 27th October
22nd Marine Mammal Biennial, Canada (conference site: link)
“An explant approach to understand adipose tissue function; metabolic profiles of blubber tissue differs between tissue depth, cell culture conditions and energetic state.”

So if you are attending either of these conferences you can catch me there too!

Safe travels!