Once again the time for the PHATS team to leave the Isle of May has come round, and this year the field season has absolutely flown by. As sad as it is to leave, we’ve had an incredibly successful time on the island and collected all the data that we were hoping to gather, which is great as this will be the last breeding season we have to work on before the PHATS project ends in August. I’m already back on the mainland as earlier in the week I brought off all the samples we collected to make sure they were safely transferred to Abertay University without getting defrosted, and the last three members of the team will be coming home tomorrow.
The colony has emptied over the last week and there are hardly any weaned seals left anymore, let alone adults. We did get to see a familiar seal face during December however, as one particular female seal came up beside our house on the island to rear her pup last year, and this year she did exactly the same! Known affectionately as the ‘Lady of the Lake’ due to her habit of going for a swim in the reservoir at the end of Fluke street (where our house is), she is a particularly laid back seal who has successfully raised two pups in that location over the last two years. We don’t know why she decided to come up the road to raise her pup so far away from the other seals, but now she’s been here for two years it would be interesting to see if she continues to return to that spot in the future, or if other female seals followed her example. How breeding seals form new colonies and why some parts of the island are really dense with seals while other parts are empty are all a mystery currently so we can’t really guess what is motivating her to chose such an unusual location to rear her pup. When studying the seals on the island, we often have to look for flipper tags to recognise them, but as grey seals have stable spotty patterns on their fur you can also use that to identify the same individual every year, if you have a picture of them. This is how we know the ‘Lady’ is the same seal, and such photo ID methods are pretty common in the marine mammal world to repeatedly identify individuals in the wild.
The PHATS team will be back out in the field in early January 2018, when we will return to the Isle of May to look for moulting grey seals that are a year or two old to study. We will resume blog updates then, so in the meantime we all wish you Merry Christmas, a Happy New Year and hopefully see you in 2018!
However, one of the few positive things about combating marine litter is that there are lots ways that everyone can make small changes in their lives to make real reductions in the plastic going into our environment. Here are just a few, try them and be part of the solution!
Get a re-usable water bottle (or coffee mug if you drink more of that than water!), and use water fountains to refil it through the day rather than buy bottled water. If there isn’t a water dispenser at your place of work, talk to your bosses to get one installed for everyone to use.
Get re-usable shopping bags to use instead of disposable ones from supermarkets.
Say you don’t need a straw at bars and restuarants.
If you don’t have any use for something you own anymore, try giving it to a charity shop, or making it into something new rather than throwing it to landfill. Coming up with creative ways to re-use things can be lots of fun, as well as saving you money and helping the environment.
If you are out for a walk and see some litter blowing around, pick it up! Then you can dispose of it properly at the next opertunity. Ever piece of trash picked up and taken to a bin is one less piece of garbage that will end up in the sea.
It’s not only litter and plastics that make up the marine debris problem. Fishing gear can also be deadly for marine life, types include:
The links between these negative impacts and microplastic exposure are still being uncovered, however it is thought that some of the problems associated with accumulating microplastics in the body may relate to the chemical pollutants held within the plastics. Several correlations between high microplastic ingestion rates and high concentrations of a variety of pollutants have been found marine species (e.g. these sea birds). However, correlations do not always equate to causality, and there are also studies showing no links between pollutant concentrations in plastic debris and pollutant burden in individuals eating the debris. One thing is sure however, microplastics in the marine environment are not going away anytime soon, and more work is needed to understand the problem and its consequences for marine organisms. The PHATS team that I am part of is working to uncover the physiological affects of persistent organic pollutants (or POPs, e.g. PCBs) on fat tissue from seals, and we need to understand how individuals get exposed to pollutants. Microplastic ingestion may represent an additional, significant route of exposure to these harmful chemicals in addition to those that are eaten when bound to the fatty tissues of prey speices, that have bioaccumulated up the food chain. Hopefully in the coming years, the mechanisms underlying POPs bound to microplastics, and their absorption into the tissues of marine organisms that ingest them, will become clearer.
Our research for the PHATS project on the Isle of May is going really well, and almost all of our study pups are now weaned from their mothers. Watching the pups go from skinny, fluffy newborns to massively fat, sleek weaners in just over two weeks is always a part of our work that fascinates me; it’s incredible that they can put on so much mass in such a short time frame. Soon the weaned pups will start leaving the island to go to sea for the first time, and their large blubber reserves will hopefully tide them over until they can learn how to fish by themselves.
As it’s getting into the late part of the season for the research team here on the island, we’ve also had lots of human comings and goings in the last week. We’ve had a film crew out from the BBC winter watch team, so hopefully footage of the Isle of May seals and some of the science done on the island will be coming to TV soon. Almost half of the research team has also returned back to the mainland, including the PHATS team leader Dr Kimberley Bennett, who has to get back to the University of Abertay to continue her lecturing duties. As the season continues, the team will probably drop to fewer than 4 people, who will stay out to finish the research work and then close up the island for the holiday season. We won’t be gone for long though, as the PHATS team are already planning our return in early January!
The first two weeks of our field season on the Isle of May have been very busy ones, and we’re now well into the research that we need to get done on the island. In the last week the colony hit peak pupping time, which meant there was lots of amazing births to watch on the island. So, in honour of all the little lives I’ve witnessed come into this world over the last 2 weeks, this blog will be about the wonders of seal pregnancies and births. Births and young pups are fascinating to observe, but please keep in mind that all the observations and photographs we take are done under permit and from hides during research on the breeding colonies as part of scientific projects. Please do not approach or disturb seals during autumn, as they may be pregnant or with pups. Mothers may abandon pups if people come too close, and then the pups will starve to death.
Seal pregnancies are very different to human ones, as they can delay implantation of the growing embryo in the womb, so that it stops developing for a certain time period before it implants and the pregnancy continues normally. This enables grey seals to give birth at the same time every year, despite mating when they leave the colony on day 18 and having pregnancies than only last about 9 months. Many species of mammals, especially carnivores, show delayed implantation, or embryonic diapause, and it is widespread in the pinniped species (seals, sea lions and walrus). However, recent research has found some seal species, like the Weddell seal that lives in Antarctica, may not have delayed implantation. New studies to understand the environmental, nutritional and population pressures driving the evolution of delayed implantation in types of seal will hopefully help us figure out why some species have this physiological adaptation and others do not.
Birth and rearing a young infant is always a testing time for a mammalian mother, and in marine mammals, newborn infants face an additional challenge to their wellbeing. Marine mammals typically have high persistent organic pollutant(POP) burdens due to their top trophic positions in the food chain and the bioaccumulation of the POPs in predators. As these substances are lipophilic (they combine with fat tissues in the body, like blubber) this means that infant marine mammals are at risk from the pollutant burden of their mothers. Some transfer of POPs occurs during pregnancy across the placenta, but once the pup or calf is born, mothers have to produce fat rich milk to feed their offspring with. To do this, mothers typically mobilise the fat reserves in their blubber, and as this tissue has high POP concentrations, these go into the milk she’s producing. By having to drink milk with elevated POP concentrations, newborn marine mammals are continuously ingesting proportions of their mother’s pollutant burden up until they wean, which can have serious negative impacts on their immune system and chance at survival. The PHATS team I’m currently working with is trying to uncover more about the physiology underlying the impact POPs have on fat tissue function and an individual’s ability to generate and utilise blubber properly. By using novel tissue culture techniques in wild breeding colony locations (LINK), we’re hoping to provide new insights and develop new methods to investigate physiological problems caused by such man-made changed to the environments.
MEANWHILE I’d like to introduce you to some of our study seals! They are all named after the phonetic alphabet, and we watch them from birth, to weaning and beyond to gather data and samples from them. This means that at any one time, we have white coat pups that are still with their mothers, pups that have just weaned and are moulting their white baby fur (or lanugo) and pups that are well into their 1-4 week post-weaning fast, with their spotty adult fur. Below are a few of our study seals, and I’ll post updates about how they are all doing on the blog every week.
This year the field season has had a rather unconventional start, as for the first week of the breeding season Dr Bennett and I were away in Canada for the 22nd Biennial Marine Mammal Conference (more on this later). So back in mid October we had to do an early provision run to get most of the laboratory gear we need for the 2 month long field season out onto the island. The other field teams working on the seals then arrived while we were still in Canada, and when we finally arrived last week we had to hit the ground running as there were already lots of potential study animals for the PHATS project. Even though we’ve only been on the island for three days, we’ve already found the first five seals for this year’s study cohort, who have duely been named Alpha through to Echo from the phonetic alphabet. We’ll be here until mid december to try and collect all the data we need to finish the PHATS study, as this is the last field season that is planned for the project.
Outside of the lab we’ve set up on the island, the breeding season is in full swing for the grey seals that have come here to give birth and mate. The number of mother-pup pairs is steadily rising and the large males are already starting to battle for position among the females. There aren’t too many weaned pups around yet, but within a few weeks there will be loads all around the edges of the colony as their 18 days with their mothers comes to an end and the females return to sea, leaving their pups to fend for themselves. Every week I’ll write about a different aspect of the breeding colony and the PHATS project for this blog, with updates on how our study seals are doing and what the field team are getting up to. You can check back here or find me on twitter for updates.
MEANWHILE as mentioned above, team leader Dr Bennett and I have been travelling, heading to Halifax, Canada for the 22nd Biennial Marine Mammal Conference to present the findings of the PHATS project so far. It’s always fantastic to get to meet up with fellow marine mammal scientists, hear what discoveries have been made in the last 2 years and show people what you’ve been working on. I also go to take part in a workshop dedicated to a subject that’s become especially important to me, marine mammal endocrinology. It was great to meet all the other people working on the challenging topic of marine mammal hormones, and to hear about the inventive ways people get around working with tricky species like whales out at sea. I’ve had a pretty busy year for conferences in 2017, hopefully we’ll keep finding out new, interesting things from both my PhD on oxytocin and from the PHATS project so we can go to some more next year!
It’s that time of year once again, autumn is here and that means I’m making inventories and packing equipment for the PHATS team’s field season on the Isle of May. We’ll be heading out to the island at the end of October to begin our last data collection season for the project, and we’ll be living on the island and studying the grey seals until mid December. Before we head out though I’ve got a busy month ahead of me, as I’ll be presenting PHATS work, my PhD work on oxytocin and talking to the public about grey seals. But before we get onto where and when I’ll be presenting, I’m quickly going to give a shout out to a new group I’ve been involved with setting up over the last few months, the SOI Early Careers Network.
This grew out of a group of friends from the Scottish Ocean’s Institute (SOI) meeting to help each other practise for presentations, to give feedback on each other’s ideas and to chat and share resources about the various issues early career scientists face. We then decided to open the gatherings up to any early career researcher at the SOI, and the group has grown ever since. We meet at least every week, sometimes more, to discuss anything our members currently need help or advice with. Right now we are having lots of conference poster and talk preparation sessions with the biologging meeting and the marine mammal biennial happening in September and October. We’ve also discussed loads of topics including statistical methods, funding awards and public outreach.
If you are an early careers researcher at the SOI you are very welcome to join us, our meetings aim to address whatever our members feel they currently need, providing a responsive support system with a relaxed, friendly environment. Please visit our new website here to find out more, see when our next meetings are and sign up to the mailing list, or you could come along to our welcome day event on Tuesday 3rd October (next week) to meet some of us and chat about the group and early career life.
I’ll certainly be practising the various presentations I need to give in the coming month at the ECN! I’ve already been to one conference this month, the wonderful meeting of the British Neuroendocrinology Society in Nottingham where I got to present my work on oxytocin and behaviour in seals. Next I’ll be talking to the public about any and all aspects of grey seal life on the Isle of May, during their annual seal weekend. This happens to celebrate the start of the grey seal breeding season, and afterwards the island is then closed to the public for the rest of the year to protect the breeding seals from disturbance.
I’ll then be travelling to the University of Edinburgh to talk about my work on oxytocin and behaviour on the 10th October. I can’t wait to meet everyone at the Centre for Integrative Physiology and hear all about their amazing research on neuroendocrinology, I got to meet a few lab members at the BNS 2017 conference and their studies on modelling oxytocin dynamics are fascinating. Finally I’ll be heading out to Canada towards the end of October to present our PHATS work at the 22nd Biennial Conference on Marine Mammals. Phew, it’s going to be a crazy month! If you’d like to know more about any of my work, feel free to say hi at any of these events, or you can keep up with me on Twitter (@KJRscience).
1st October – Isle of May seal weekend
10th October – 3pm talk at the Centre for Integrative Physiology, University of Edinburgh
22nd-27th October – 22nd Biennial Conference on Marine Mammals, Halifax, Canada
I have now returned from an incredibly successful week at Behaviour 2017 (link), and what a spectacular conference it was! The sheer variety of science that people were talking about was incredible and inspiring, plus I got a great response to both my symposium talk on seal oxytocin and the poster I presented on aggression. I meet so many wonderful people, heard lots of interesting talks and I even managed to avoid getting roasted in the blazing Portuguese sun! I had never previously been to a behaviour conference of any kind, but this one has really encouraged me to keep an eye out for future ASAB meetings to present at. Huge thanks to the lovely people working as part of the SoHaPi research group for inviting me to speak at your symposium, I look forward to meeting up with you all in the future!
More good news was waiting for me when I arrived home from Portugal; our PHATS team leader, Dr Kimberley Bennett, let us know that the first paper the PHATS team have worked on was coming out at last! This paper details our work investigating whether an explant approach (basically blobs of many living cells) would work for culturing fat (or adipose) cells collected from wild animal species in field conditions. Additionally, we wanted to know whether we could manipulate the explants during culture to
uncover the physiological consequences of changes in the nutrients or hormones the cells have access to. We found we could not only keep our cells alive once collected from wild seals on the coast of Scotland, but once transported back to the lab we could culture the cell explants for at least 24 hours. During this time we could expose the adipose cells to different treatments, such as high glucose concentrations in the cell culture media (the sugary, salty goo that cells are suspended in during culture to keep them alive) or difference hormone additions, such as hydrocortisone. We found significant differences in the metabolic profiles of adipose cells given different treatments, demonstrating this technique could be used to test the responses of wild animal tissue to a variety of substrates an individual may physiologically generate, or be exposed to.
Studying wildlife physiology is always challenging because collecting samples is tricky, typically giving small samples sizes in less than ideal conditions for complex labwork. However our work to bring cell culture techniques to the wild regions of Scotland shows that even difficult processes like cell culture, which require sterile conditions, aseptic technique and specialised equipment, are possible with thought and preparation beforehand. Studying cell function in wild animals is important as how different tissues function in response to different environmental challenges will impact on how individuals survive. Fat tissue is especially crucial for survival as it represents the energy stores animals have to rely on when conditions are tough, and also helps keep individuals warm in cold environments. By understanding how fat tissue functions, we can better understand why different species in changing environments can either adapt to meet new energetic challenges or be overwhelmed by them.
Speaking of ‘the wild regions of Scotland’, it’s that time of year when I start prepping all the field equipment for the PHATS team’s annual research trip to the Isle of May grey seal breeding colony, off the east coast of Scotland. Join us here for our fieldwork blog, bringing you all the adventures we have running a tissue culture lab on an island full of seals. We are scheduled to leave in late October, and will stay on the island studying the seals for about 2 months, heading home just in time for Christmas (hopefully!). I’ve also got two more conferences to attend before I go off into the field, one in September in Nottingham, UK with the British Society for Neuroendocrinology and one in October in Halifax, Canada with the Society for Marine Mammalogy. If you are going to either and want to say hello I look forward to meeting you there!
Just like that, my time at the University of Liege has finished and I’m back at the Sea Mammal Research Unit in Scotland. I was successful in preparing all our grey seal blubber samples for analysis, and now we just need to wait for the results from the Gas Chromatography – Mass Spectrometry (GC-MS)
machines. We will hopefully get all our results by July, and in the meantime I will get back to the bichemical analysis of the samples from the tissue culture experiments on the Isle of May last year. My last few days in Liege flew by, a whirl of labwork, tasty Belgian fries and one last trip to Masion du Peket to enjoy their delicious drinks!
We’re not just focusing on labwork here on the PHATS team however. We’ve been working hard on analysing our data and are now ready to start getting our science out there! We’ll hopefully be attending conferences this year to present our findings, and if you are interested in our work do come and find us at the below venues. I will also hopefully be presenting some of my work on the hormone oxytocin and it’s affects on bonding, social and maternal behaviour in seals. While the blog will be on hiatus until we return to the field in October, we will update it when we attend or present at conferences, or if we publish any papers on our work so watch this space!
30th July – 4th August: Behaviour 2017 (ASAB summer meeting & 35th International Ethological Conference)
My last week at the University of Liege has arrived, and I’m working hard to ensure that all the PHATS team’s labwork is complete before I leave to return home. All of our samples are now ready for analysis that will let us detect PCB and PBDE levels in our Scottish grey seals. PCBs and PBDEs are two types of the many persistent organic pollutants (POPs) that are present in our environment. I am currently working on preparing our samples for another type of analysis that will enable us to detect a third kind, OCPs. As POPs in our environment, and PCBs in particular, are still currently in the news after the recent revelation of just how highly contaminated with PCBs some marine mammals are becoming, I thought I’d spend this blog introducing the three types of POP I work on and why they are so problematic.
PCBs, or polychlorinated biphenyls, are pollutants that are made up of two linked rings of carbon atoms with a varying number of hydrogen and chlorine atoms bound to the rings at different positions. There are many possible combinations of the number and locations of the hydrogen and chlorine atoms binding to the rings, and these give rise to the large variety of PCBs (called congeners) that exist. Approximately 130 different types of PCB are found in commercial products, and they can be divided into two groups (dioxin-like and non-dioxin-like) based on their structure and toxicity. PCB production was banned in the USA in 1979 and by the Stockholm convention (signed by over 150 countries worldwide) in 2001, however they persist in our environment due to their slow degradation rates. One of the main reasons PCBs were previously manufactured and used in industry was their inert properties; only incineration at high temperatures can safely destroy them. Previous uses of PCBs include in coolants and lubricating oils, paints and electric wire coatings.
PBDEs, or Polybrominated diphenyl ethers, are also made up of two carbon rings, but they have bromine bound to the rings rather than chlorine. The fewer the bromine atoms per molecule of PBDE, the more dangerous they are considered to be as congeners with between 1-5 bromine atoms bioaccumulate more effectively in living organisms. PBDEs are still being manufactured and widely used in many man-made products, the Stockholme convention which banned PCBs only restricted the production of some PBDEs. Some states in the USA have begun prohibiting their manufacture and use in the last decade however. PBDEs are flame retardant and are therefore commonly incorporated into electronics, plastics, fabrics and other building materials.
OCPs, or organochlorine pesticides, contain carbon, hydrogen and at least one bound chlorine atom but do not contain carbon ring structures like PCBs and PBDEs. There are many different types of OCP, however arguably the most well known is DDT (Dichlorodiphenyltrichloroethane) which was heavily used as a pesticide across the world to kill insects for both agricultural and disease control purposes. The famous book ‘Silent Spring’, written by Rachel Carson in the 1960s, is all about OCPs and the negative impact overuse of pesticides has on the environment. The production and use of some OCPs like DDT and heptachlor has been strictly limited by the Stockholme convention. Due to their efficiency at killing insects, their use is still permitted in some circumstances, such as the use of DDT to control mosquitoes that carry diseases like malaria.
POPs have been connected to a wide range of negative health impacts in both people and wildlife, and chronic exposure to any type of POP will cause problems for any organism. All POPs are carcinogenic (cancer causing) and are potent endocrine disruptors, interfering with growth and development, immune function and reproductive systems. There is growing evidence that POPs impact on obesity, leading them to be labelled as ‘obesogens’. The PHATS project I am part of is hoping to uncover some of the underlying physiological and genetic mechanisms that influence fat tissue function and determine how POPs can interfere with these processes. By studying a marine mammal species which has lots of fat and lots of bioaccumulated POPs, we can gain a better understanding of how these chemicals have such far reaching and devastating impacts on our health and the environment.
It’s been another busy week here in chemistry labs at the University of Liege. I’ve completed extracting all the PHATS team’s blubber samples for persistent organic pollutant (POP) analysis, and now am moving on to the purification part of the sample preparation process. I’ve only got two weeks left to get all the sample preparation completed, so hopefully all the lab work will go according to plan! The purification process isn’t too complicated but it does have lots of time consuming steps, from multiple standard spikes, to acid clean-up on columns, to concentrating the samples down via nitrogen evaporation. So it’s just a case of getting your head down and getting on with it all, as the sooner it’s done the sooner we’ll have some interesting results to look through.
The results of POP studies are frequently worrying as well as interesting. A good example of this happened last week, when the Scottish Marine Animal Stranding Scheme (SMASS) got some lab results back showing the PCB concentrations in one of the stranded whales they had examined last year, ‘Lulu’, one of Scotland’s few resident orca. She sadly had one of the highest ever recorded concentrations of PCBs in her body, and there are concerns that the other members of her pod will have similarly high levels. Another interesting (and sad) aspect of Lulu’s case is that she had never produced a calf, despite the fact she was about 20 years old and orca usually have their first calves at around 14 years of age. It is well known that POPs negatively impact on individual health, including fertility, therefore it is possible Lulu failed to reproduce due to her high pollutant burden. Even more concerning however, is what might have happened to Lulu’s high PCB concentrations if she had produced a calf.
Another major cause of patterns in POP concentrations in marine mammals is their position in the food chain (their trophic level) and the region they obtain their food from. Orca represent a fascinating opportunity to study these patterns as through-out the species, there are different populations that specialise in eating either fish or other marine mammals, or in other words different orca populations can occupy different tropic levels of a food chain. Groups that eat marine mammals, such as seals, sea lions and porpoises, typically have over double the concentrations of POPs in them than fish eating groups. This happens because the pollutants have become concentrated up the food chain due to bioaccumulation, where a predator eating lots of smaller prey gets all the pollutants in each individual it eats. A whale eating lots of seals to survive will accumulate all the pollutants all the seals were exposed to, and all the pollutants all the fish those seals ate too. Meanwhile a fish eating individual will ‘only’ accumulate the pollutants from the fish it eats. Additionally, individuals that hunt in highly industrialised areas have higher concentrations than those in ‘pristine’ areas, because the more POPs that are in a local area, the higher the concentrations in all the organisms from the bottom of the food chain to the top.
Studying patterns of POP concentrations in different types of individuals can therefore lead to a better understanding of how these persistent pollutants ‘move’ through organisms and can be transferred into later generations. It is not hard to see why POPs continue to be a problem for animal and human health, despite being banned decades ago.
Well my first week at the University of Liege working with CART has flown by, and I’ve been working on the blubber biopsies we collected from the grey seals last year on the Isle of May. All the lipids (fats) need to be extracted from the blubber tissue before we can move forward with the pollutant analysis, so all the samples must be carefully prepared and put through Accelerated Solvent Extraction (ASE). This process uses high pressure and temperature conditions plus chemicals called solvents (like hexane and acetone) to remove all the lipids from the sample in the cells. This process gives us a completely liquid solution of lipids and solvents at the end of it, and we can then evaporate the solvent to leave just the lipids from our sample. This step is important as it gives the lipid mass of our sample, and allows us to work out how many nanograms of pollutant per gram of lipid in our sample there is (ng/g) . While ASE of our samples is an important step in the lab work, there isn’t really much more to say about it so I’m going to use the rest of the blog this week to give a brief introduction to blubber tissue, a crucial part of the anatomy of all marine mammal species worldwide.
All marine mammals, from the largest whale to the smallest seal, have a layer of fat underneath their skin called blubber. This layer of fat is extremely important for the survival of marine mammals for two reasons:
It enables them to keep warm (thermoregulate) in freezing oceans.
It provides a store of energy for individuals to utilise when they are not feeding, which happens in many marine mammal species at various points throughout their lives due to breeding or moulting.
Fat tissue deposits in all animal species perform these same two functions, however other species frequently have additional ways to thermoregulate (such as fur in land mammals) or do not endure long periods of fasting repeatedly while migrating or breeding as many marine mammals do. The importance of this tissue has lead to substantial blubber thickness evolving in marine mammals, and a stratified structure throughout the depth of the tissue is present so that it can perform both functions at the same time. Typically, blubber tissue can be roughly divided into three sections as you go from the part closest to the skin (the outer blubber) to the part closest to the inside of the seal (the inner blubber). The inner blubber is the most metabolically active, and this is where lipids are mobilised to provide energy for an individual when it either cannot find food or is purposefully fasting. The mid blubber is the most variable in thickness across individual marine mammals, and in thin individuals can be completely absent. It is thought it acts as a more long term storage tissue, and that its thickness is influenced by seasonal food availability. The outer blubber is typically of stable thickness within a species regardless of the nutritional state of an individual, and is thought to be primarily for thermoregulation. Hence even starving individuals will always have some blubber tissue to keep them from freezing, as the outer blubber is not mobilised as an energetic resource.
Blubber is a fascinating tissue to study and several different approaches can be used to analyse it in many contexts, like this recent study by one of my friends, Joanna Kershaw, who measured the hormone cortisol in blubber from porpoises to validate it’s use as a biomarker of body condition. The PHATS project I work on uses both established techniques (investigating pollutant concentrations) and novel protocols (the explant approach for tissue culture experiments that our team leader pioneered in seals) to make the most of the blubber samples we collect from our study animals to explore the prevalence of persistent organic pollutants in the marine environment and it’s impact on energy balance in seals.
MEANWHILE I am settling back into Liege life quite happily outside of the lab. I am not staying in the university accomodation this year, and have a lovely little flat not far from the campus to retreat to. In my time away from the lab I’m trying to keep up with the usual paper and grant writting that all resarchers need to keep on top of, plus greatly enjoying bebing reunited with the amazing macaroons they make here! Seriously, why can’t they be this good in Scotland…